Calgary Metabolomics Research Facility (CMRF) Instrumentation
The CMRF is a $4 million facility designed specifically for investigating the metabolism of infectious diseases. This facility includes an integrated Biosafety Level 2 microbiology laboratory, engineering space, and mass spectrometry lab to facilitate research and development of new diagnostic devices and analytical strategies. The CMRF’s dedicated staff provide expertise in high throughput metabolomic and proteomic analyses of a wide variety of complex biological samples.
High Resolution Metabolomics
Q-Exactive HF
Thermo Fisher Scientific
Thermo Fisher Scientific Q-Exactive HF coupled to a Vanquish Horizon UHPLC, configured for:
Untargeted analysis
Semi-targeted detection of common microbial nutrients, media components, and waste products
Metabolic boundary flux analysis
Thermo-Fisher Scientific Q-Exactive coupled to a Vanquish Flex UHPLC (configured for ion-pairing chromatography), configured for:
Semi-targeted detection of intercellular and central carbon metabolites
Isotope tracing
Metabolic boundary flux analysis
Q-Exactive
Thermo Fisher Scientific
Targeted Metabolomics
Altis
Thermo Fisher Scientific
Thermo Fisher TSQ Altis (QqQ) coupled to a Vanquish Flex UHPLC configured for:
Targeted quantification of metabolic biomarkers
Targeted analysis of microbial metabolites in large cohort studies
Thermo Fisher TSQ Quantum Access Max (QqQ) coupled to a Vanquish Flex UHPLC configured for:
SCFA quantification
Targeted quantification of metabolites of interest
Quantum Access Max
Thermo Fisher Scientific
Fortis
Thermo Fisher Scientific
Thermo Fisher TSQ Fortis (QqQ) coupled to a Vanquish UHPLC configured for:
Targeted lipidomics quantification
Metabolic engineering
High Resolution Proteomics
Thermo Fisher Fusion Lumos coupled to an EASY-nLC 1200 System and FAIMS configured for:
TMT labelling based proteomic workflows on large cohorts microbial datasets
Fusion Lumos
Thermo Fisher Scientific
Support Instrumentation
Genomics
Hamilton Robotics Microlab NIMBUS
Automatized sample preparation for genomic and proteomic workflows
Automatization
Computation
